Synopses & Reviews
New and exciting biological functions are still being discovered for vitamin A derivatives, including the vast number of physiological activities of retinoids. In Retinoids: Methods and Protocols, expert researchers in the field present the most recent technical tools with diverse techniques for both in vitro and in vivo studies. Combining biochemical, biophysical, and cell biological techniques, the book addresses topics such as the detection and quantitation of retinoids using HPLC, mass spectrometry, and fluorescence, fluorescence anisotropy of retinol binding protein, cell culture models for studying retinoid transport and the role of retinol in embryonic stem cell culture, as well as many other detailed procedures. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes highlighting tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Retinoids: Methods and Protocols seeks to aid beginning and experienced researchers from widely varied fields in the search to uncover even more vital aspects of vitamin A's impact on the human body.
Exciting new biological functions are still being discovered for vitamin A derivatives, including the myriad physiological activities of retinoids. Here, experts in the field present the latest range of techniques for both in-vitro and in-vivo studies.
Table of Contents
1. Quantification of Endogenous Retinoids Maureen A. Kane and Joseph L. Napoli 2. Culture of Highly Differentiated Human Retinal Pigment Epithelium for Analysis of the Polarized Uptake, Processing, and Secretion of Retinoids Jane Hu and Dean Bok 3. Feeder Independent Culture of Mouse Embryonic Stem Cells Using Vitamin A/Retinol Jaspal S. Khillan and Liguo Chen 4. In Vitro Assays of Rod and Cone Opsin Activity: Retinoid Analogues as Agonists and Inverse Agonists Masahiro Kono and Rosalie K. Crouch 5. Physiological Studies of the Interaction between Opsin and Chromophore in Rod and Cone Visual Pigments Vladimir J. Kefalov, M. Carter Cornwall, and Gordon L. Fain 6. Measurement of the Mobility of All-Trans Retinol with Two-Photon Fluorescence Recovery after Photobleaching Yiannis Koutalos 7. Microfluorometric Measurement of the Formation of All-Trans Retinol in the Outer Segments of Single Isolated Vertebrate Photoreceptors Yiannis Koutalos and M. Carter Cornwall 8. HPLC/MSN Analysis of Retinoids James E. Evans and Peter McCaffery 9. Binding of Retinoids to ABCA4, the Photoreceptor ABC Transporter Associated with Stargardt Macular Degeneration Ming Zhong and Robert S. Molday 10. Fluorescence-Based Technique for Analyzing Retinoic Acid Leslie J. Donato and Noa Noy 11. The Interaction Between Retinol-Binding Protein and Transthyretin Analyzed by Fluorescence Anisotropy Claudia Folli, Roberto Favilla, and Rodolfo Berni 12. Assay of Retinol Binding Protein-Transthyretin Interaction and Techniques to Identify Competing Ligands Nathan L. Mata, Kim Phan, and Yun Han 13. Molecular Biology and Analytical Chemistry Methods Used to Probe the Retinoid Cycle Marcin Golczak, Grzegorz Bereta, Akiko Maeda, and Krzysztof Palczewski 14. Visualization of Retinoid Storage and Trafficking by Two-Photon Microscopy Yoshikazu Imanishi and Krzysztof Palczewski 15. Reverse-Phase High Performance Liquid Chromatography (HPLC) Analysis of Retinol and Retinyl Esters in Mouse Serum and Tissues Youn-Kyung Kim and Loredana Quadro 16. Detection of Retinoic Acid Catabolism with Reporter Systems and by In-Situ Hybridization for CYP26 Enzymes Yasuo Sakai and Ursula C. Dräger 17. Diet in Vitamin A Research A. Catharine Ross 18. Experimental Approaches to the Study of A2E, a Bisretinoid Lipofuscin Chromophore of Retinal Pigment Epithelium Janet R. Sparrow, So Ra Kim, and Yalin Wu 19. Analysis of the Retinoid Isomerase Activities in the Retinal Pigment Epithelium and Retina Gabriel H. Travis, Joanna Kaylor, and Quan Yuan 20. Techniques to Study Specific Cell-Surface Receptor-Mediated Cellular Vitamin A Uptake Riki Kawaguchi and Hui Sun