Synopses & Reviews
The authors describe the main large-scale bioprocessing strategies for both stem cell culture and purification, envisaging the application of these cells for regenerative medicine and drug screening. They also explain bioreactor configurations, including their applications for stem cell expansion and/or differentiation, and discuss stem cell separation techniques, which aim towards both isolation and purification of rare stem cell populations. For a better understanding of these processes, they provide basic definitions for the different types of stem cells, from adult stem cells to the more recent and promising induced pluripotent stem cells. The authors also describe the main characteristics of these different stem cell types, alongside the molecular mechanisms underlying their self-renewal and differentiation. There is also a focus on methodologies currently used for in vitro stem cell culture under static conditions, including the challenge of xeno-free culture conditions, and culture parameters that influence stem cell culture. Chapters present novel approaches for both stem cell culture and separation in micro-scale conditions, including the use of cellular microarrays for high-throughput screening of the effect of both soluble and extracellular matrix molecules and the use of microfluidic devices for both stem cell culture and separation. The conclusion explores application of stem cells for regenerative medicine and discusses on-going clinical trials.
Stem cell bioprocessing describes the main large-scale bioprocessing strategies for both stem cell culture and purification. It envisages the application of these cells for regenerative medicine and drug screening. Chapters describe bioreactor configurations and stem cell separation techniques and provide basic definitions and main characteristics of the different types of stem cells, alongside the molecular mechanisms underlying their self-renewal and differentiation. The book also focuses on methodologies currently used for in vitro stem cell culture under static conditions including the challenge of xeno-free stem cell culture. Final chapters present approaches for stem cell culture and separation in micro-scale conditions, with a separate section dedicated to the application of stem cells for regenerative medicine.
About the Author
Dr. Tiago G. Fernandes is currently a Post-doctoral research fellow at the Institute for Biotechnology and Bioengineering, Lisbon, Portugal. His research interests are focused on the development of artificial cellular niches for studying the mechanisms that affect human stem cell pluripotency, and aim at contributing to a better understanding of the cellular and molecular events that regulate stem cell functions. During his doctoral studies he also worked at the Rensselaer Polytechnic Institute, New York, in the development of microscale platforms for high-throughput studies of stem cell fate.
Dr. Maria Margarida Diogo is presently a Research Scientist at the Stem Cell Bioengineering Laboratory at Instituto Superior Tecnico, Portugal.
Dr. Joaquim M. S. Cabral is Professor of Biological Engineering in the Department of Bioengineering at Instituto Superior Tecnico (IST), Technical University of Lisbon, Portugal. He was the founder and is the current Head of the Department of Bioengineering and the Director of the Institute for Biotechnology and Bioengineering, a Research Laboratory Associated to the Ministry of Science, Technology and Higher Education in Portugal.
Table of Contents
Definitions; Sources: Pluripotent stem cells: Embryonic stem cells; Several sources; Induced pluripotent stem cells; Reprograming techniques: Viral vectors; Non-viral vectors; Adult stem cells; Molecular mechanisms: Systems biology
Stem cell culture
Culture parameters: Culture medium; Feeder cell layers; Oxygen tension; Osmolarity
Bioreactors for stem cell culture
Bioreactor configurations; Bioreactors for stem cells; Kinetic modelling of stem cell growth and differentiation
Stem cell bioseparation Stem cell isolation and purification; Separation operations (ATPS, Chromatography, membrane separation, MACS, FACS); Quality control after stem cell bioprocessing
Micro-scale technologies for stem cell culture and separation
Micro-scale for stem cell culture: Microarrays; Microfluidics; Micro-scale for stem cell separation: Screening platforms: Drug screening and toxicology; Stem cell research
Stem cells and regenerative medicine
Stem cells and cellular therapy; Clinical trials