Synopses & Reviews
The intra-cellular trafficking field has made a quantum leap in the last few decades. The three sections of this book cover the past, present and future of this rapidly developing field. The first section is about the compartments and pathways defined more than 50 years ago by the pioneering studies of George Palade, who received the Nobel Prize for this work. However, as shown in the chapters in this section, new approaches that allow us to study the dynamics of these compartments and pathways have revealed that the compartments are not as stable as was previously thought. Several issues discussed within this section are still controversial. The second section on mechanisms is what the field has been focused on during the last 20-25 years. This is the largest section of the book. Starting with the work of James Rothman and Randy Schekman, components of the machinery were identified and mechanisms were deciphered. Using in vivo and in vitro approaches combined with genomics and proteomics, the highly conserved molecular machines that move vesicles between cellular compartments are being characterized. This phase is also not complete yet, but a clear picture is beginning to emerge. Based on the foundation of the pathways and the machinery components, the field is now embarking on understanding how individual transport steps are regulated, how successive steps are integrated into whole pathways, and how these pathways are coordinated with other cellular processes. The third section belongs to the future, and addresses issues of increasing importance in this area.
Synopsis
The human body is made up of trillions of tiny cells that cannot be seen by the naked eye. The functioning units inside these cells are macromolecules that need to travel in the three-dimensional cell-space to distances ten thousand times their size. This movement is highly ordered, requires energy and takes place on molecular tracks that serve as a sophisticated transport system--somewhat equivalent to the multimodal rail-highway-river networks of large metropolises. All the systems of the human body depend on the efficient delivery of macromolecules to their right destination at the right time--both within and between cells. Breakdown of this traffic system results in a variety of diseases including diabetes, cancer and heart disease, as well as immunological, neurological and developmental disorders. During the last half a century, scientists have made a quantum leap in unraveling the mysteries of trafficking inside cells. The three sections of this book together cover the past, present and future of this rapidly developing and intriguing field.
Synopsis
This book covers the past, present and future of the intra-cellular trafficking field, which has made a quantum leap in the last few decades. It details how the field has developed and evolved as well as examines future directions.
Table of Contents
Section I. Compartments and Pathways
1. Overview of Intracellular Compartments and Trafficking Pathways
Andrei A. Tokarev, Aixa Alfonso and Nava Segev
2. How We Study Protein Transport
Mary L. Preuss, Peggy Weidman and Erik Nielsen
3. The Golgi Apparatus
Zhaolin Hua and Todd R. Graham
4. The Endocytic Pathway
Elizabeth Conibear and Yuen Yi C. Tam
5. Regulated Secretion
Naveen Nagarajan, Kenneth L. Custer and Sandra Bajjalieh
Section II. Mechanisms
6. Overview of Protein Trafficking Mechanisms
Giancarlo Costaguta and Gregory S. Payne
7. Entry into the Endoplasmic Reticulum: Protein Translocation, Folding and
Quality Control
Sheara W. Fewell and Jeffrey L. Brodsky
8. COP-Mediated Vesicle Transport
Silvere Pagant and Elizabeth Miller
9. Clathrin-Mediated Endocytosis
Peter S. McPherson, Brigitte Ritter and Beverly Wendland
10. Biogenesis of Dense-Core Secretory Granules
Grant R. Bowman, Andrew T. Cowan and Aaron P. Turkewitz
11. Lipid-Dependent Membrane Remodelling in Protein Trafficking
Priya P. Chandra and Nicholas T. Ktistakis
12. Carrier Motility
Marcin J. Wozniak and Victoria J. Allan
13. Tethering Factors
Vladimir Lupashin and Elizabeth Sztul
14. Intracellular Membrane Fusion
Dalu Xu and Jesse C. Hay
Section III. Regulation and Coordination with Other Cellular Processes
15. Regulation and Coordination of Intracellular Trafficking: An Overview
Julie Donaldson and Nava Segev
16. Regulation of Protein Trafficking by GTP-Binding Proteins
Michel Franco, Philippe Chavrier and Florence Niedergang
17. Posttranslational Control of Protein Trafficking in the Post-Golgi Secretory
and Endocytic Pathway
Robert Piper and Nia Bryant
18. Actin Doesn't Do the Locomotion: Secretion Drives Cell Polarization
Mahasin Osman and Richard A. Cerione
19. Intracellular Trafficking and Signaling: The Role of Endocytic Rab GTPase
M. Alejandro Barbieri, Marisa J. Wainszelbaum and Philip D. Stahl
20. The Exocytic Pathway and Development
Hans Schotman and Catherine Rabouille