Synopses & Reviews
Now in its sixth edition, Principles of Gene Manipulation provides an excellent introduction to the area of genetic engineering of plants, animals and microbes for advanced level undergraduates, with a basic understanding of genetics. This classic textbook has been substantially updated and revised to reflect the rapid advances that have been made in the core technologies in the seven years since the last edition. Furthermore, to put these technologies into context, the final chapter has been structured into six themes:
· nucleic acids as diagnostic tools
· new drugs and new therapies for genetic diseases
· combating infectious disease
· protein engineering
· metabolic engineering
· modern plant breeding
A website is now available to complement this text, at www.blackwellpublishing.com/primrose
Review
From the fifth edition: "This book perpetuates the quality and utility which have made its previous editions probably the best introductory text on gene manipulation of the past decade and a half: a generation of our students have relied on it."
Journal for the Society for General Microbiology,1995 "The authors are to be congratulated for their courageous effort to bring the new edition up-to-date in an area that is a technological hallmark of the 21st century[...]The starting level is still well suited to advanced undergraduates, but it quickly leads to specialized sophisticated technologies and applications[...]overall, the scientific content of this book is seriously impressive, its coverage comprehensive and it will undoubtedly become an important resource to students, course organizers and young scientists" Irina R. Tsaneva MICROBIOLOGY TODAY August 2002
"Now in its sixth edition, Principles of Gene Manipulation is obviously quite well-honed and serves the needs of its traditional target readership - advanced undergraduates or early postgraduates - extremely well. It is clear that it would be equally useful to other types of reader requiring lucid and well-balanced descriptions of what can now be done with genes." C David O'Connor, University of Southampton, Heredity, November 2003
Table of Contents
Preface.
The Genetic Code and Single-Amino Acid Designations.
Conversion Scale.
1. Gene Manipulation: An All-Embracing Technique.
2. Basic Techniques.
3. Cutting And Joining DNA Molecules.
4. Basic Biology Of Plasmid And Phage Vectors.
5. Cosmids, Phasmids And Other Advanced Vectors.
6. Cloning Strategies.
7. Sequencing And Mutagenesis.
8. Cloning In Bacteria Other Than E. Coli.
9. Cloning In Saccharomyces Cerevisiae And Other Fungi.
10. Gene Transfer To Animal Cells.
11. Genetic Manipulation Of Animals.
12. Gene Transfer To Plants.
13. Advances In Transgenic Technology.
14. Applications Of Recombinant DNA Technology.
References.
Index.