Synopses & Reviews
Recent advances in genetics, synergized by many exciting developments in cellular and molecular biology, have provided powerful new experimental tools for the study of not only B-lymphocyte development and function, but also the physiology and pathology of the immune system in higher animals. In B Cell Protocols, highly qualified investigators who are either the inventors or expert users of their methods provide a diverse collection of protocols that range from the latest inventions and applications to classic, but still frequently used, methods in B cell biology. The protocols presented follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, B Cell Protocols offers both beginning and experienced researchers alike highly effective tools for exploring B-lymphocyte development and function in higher animals, as well as critical information on how best to design or modify an experimental approach that will prove productive in their own research.
Synopsis
A diverse collection of protocols ranging from the latest inventions and applications to some classic, but still frequently used, methods for studying B-lymphocyte development and function in higher animals. These readily reproducible protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Table of Contents
Characterization of B Lymphopoiesis in Mouse Bone Marrow and Spleen Richard R. Hardy and Susan A. Shinton Characterization of B-Cell Maturation in the Peripheral Immune System Rita Carsetti Identification of B-Cell Subsets: An Exposition of 11-Color (Hi-D) FACS Methods James W. Tung, David R. Parks, Wayne A. Moore, Leonard A. Herzenberg, and Leonore A. Herzenberg Analysis of B-Cell Life-Span and Homeostasis Irmgard Förster Differentiation of B Lymphocytes From Hematopoietic Stem Cells Paulo Vieira and Ana Cumano Analysis of Lymphocyte Development and Function Using the RAG-Deficient Blastocyst Complementation System Faith M. Young, Carl A. Pinkert, and Andrea Bottaro Conditional Gene Mutagenesis in B-Lineage Cells Stefano Casola Analysis of the Germinal Center Reaction and In Vivo Long-Lived Plasma Cells Kai-Michael Toellner, Mahmood Khan, and Daniel Man-Yuen Sze T-Cell-Dependent Immune Responses, Germinal Center Development, and the Anaysis of V-Gene Sequences Claudia Berek Introduction of Genes Into Primary Murine Splenic B Cells Using Retrovirus Vectors Kuo-I Lin and Kathryn Calame Immunoglobulin Class Switching: In Vitro Induction and Analysis Sven Kracker and Andreas Radbruch In Vitro and In Vivo Assays of B-Lymphocyte Migration Chantal Moratz and John H. Kehrl Analysis of Antigen-Specific B-Cell Memory Directly Ex Vivo Louise J. McHeyzer-Williams and Michael G. McHeyzer-Williams B-Cell Signal Transduction: Tyrosine Phosphorylation, Kinase Activity, and Calcium Mobilization Tilman Brummer, Winfried Elis, Michael Reth, and Michael Huber Isolation of Lipid Rafts From B Lymphocytes Anu Cherukuri, Shiang-Jong Tzeng, Arun Gidwani, Hae Won Sohn, Pavel Tolar, Michelle D. Snyder, and Susan K. Pierce Molecular Single-Cell PCR Analysis of Rearranged Immunoglobulin Genes As a Tool to Determine the Clonal Composition of Normal and Malignant Human B Cells Ralf Küppers Analysis of B-Cell Immune Tolerance Induction Using Transgenic Mice Helen Ferry and Richard J. Cornall Analysis of B-Cell Signaling Using DT40 B-Cell Line Tomoharu Yasuda and Tadashi Yamamoto B-Cell Development and Pre-B-1 Cell Plasticity In Vitro Antonius G. Rolink Index