Synopses & Reviews
While many excellent texts and monographs exist which cover various aspects of immunohistochemistry, the lack of a concise comprehensive guide to using the field's methods was a major motivation for the authors to write this book. The authors carefully selected the methods and easy-to-adopt protocols discussed. In addition to estabished techniques, special attention was paid to current developments in immunohistochemistry such as antigen retrieval, signal amplification, the use of epitope tags, multiple immunolabeling, and diagnostic immunohistochemistry. All procedures examined have been tested by the authors; many are routinely used in daily practice in their institute. Each chapter starts with a short introduction to the respective method's underlying principles before presenting step-by-step protocols and instructions, including precise recipes for all materials needed. Thus, the book is suitable for a broad audience, ranging from students and technical assistants to experienced researchers.
Review
From the reviews: "Immunohistochemistry: Basics and Methods, a new book that aims to make the technique accessible to a wide audience. ... The text is easy to read and practical ... . this book is an effective primer on the science and technique of immunohistochemistry. I would recommend ... particularly to those who are themselves doing a significant amount of hands-on immunohistochemistry or for those seeking to develop the technique in their department. Indeed anyone involved in the interpretation of immunohistochemistry would benefit from reading this book." (Colan Ho-Yen, ACP News, Spring, 2011)
Synopsis
This concise yet comprehensive guide to the methods and protocols of immunohistochemistry covers established techniques and current developments in the field such as the use of epitope tags, multiple immunolabeling and diagnostic immunohistochemistry.
Table of Contents
Antibodies for immunohistochemistry; Antibody labeling and the choice of the label; Probes processing for immunohistochemistry; Working with antibodies; Background staining, autofluorescence and blocking steps; Immunostaining enhancement; Multiple multicolour immunoenzyme staining; Multiple immunofluorescence staining; Antigen detection on tissues using primary antibody raised in the same species; Probes for staining specific cellular organelles; The use of epitope tags in histochemistry; Immunohistochemistry at the ultrastructural level; Diagnostic immunohistochemistry ; A picture is worth a thousand words; Glossary