Synopses & Reviews
The second edition of Adhesion Protein Protocols combines traditional techniques with cutting-edge and novel techniques that can be adapted easily to different molecules and cell types. The topics discussed in this updated second edition include novel techniques for studying cell-cell adhesion, neutrophil chemotaxis, in vitro assays used to study leukocyte migration through monolayers of cultured endothelial cells, and novel techniques to purify pseudopodia from migratory cells. This book also discusses the study of cell-matrix interaction, RNA interference, fluorescence recovery after photobleaching, actin purification, the application of microarray techniques, and the role of adhesion proteins in the study of proteomics. The protocols discussed in this volume are suitable for both novice and expert scientists, who will gain further insight into the complex and incompletely understood processes involved in cellular adhesion.
Synopsis
An international corps of expert investigators describe their optimized techniques for both the identification of new cell adhesion proteins and for the characterization of novel adhesive structures. This edition of Adhesion Protocols combines traditional techniques with cutting edge and novel techniques that can be easily adapted to different molecules and cell types.
Table of Contents
Methods in Molecular Biology Volume 96: Adhesion Protocols Editor: Dr Amanda S. Coutts Table of Contents Preface 1. Manipulation of cell-cell adhesion using bowtie-shaped microwells. Celeste M. Nelson, Wendy F. Liu, and Christopher S. Chen. 2. Separation of cell-cell adhesion complexes by differential centrifugation. Roger Vogelmann and W. James Nelson. 3. Analysis of neutrophil chemotaxis. Paul A. Nuzzi, Mary A. Lokuta, and Anna Huttenlocher. 4. Analysis of leukocyte migration through monolayers of cultured endothelial cells. Helen M. McGettrick, Lynn M. Butler, and Gerard B. Nash. 5. Biochemical purification of pseudopodia from migratory cells. Yingchun Wang and Richard L. Klemke. 6. Dynamic assessment of cell-matrix mechanical interactions in 3-D culture. W. Matthew Petroll. 7. Quantitative Analyses of Cell Adhesion Strength. Nathan D. Gallant and Andrés J. García 8. Using RNA interference to knockdown the adhesion protein TES. Elen Griffith. 9. Assaying calpain activity. Neil O. Carragher. 10. Analysis of focal adhesions and cytoskeleton by custom microarray. Matthew J. Dalby and Stephen J. Yarwood. 11. Proteomic analysis of cell surface membrane proteins in leukemic cells. Robert S. Boyd, Martin J.S. Dyer, and Kelvin Cain. 12. Bioinformatic analysis of adhesion proteins. Josephine C. Adams and Juergen Engel. 13. Analysis of integrin dynamics by Fluorescence Recovery after Photobleaching. Bernard Wehrle-Haller. 14. Double-Hydrogel Substrate as a Model System for 3D Cell Culture. Karen A. Beningo and Yu-li Wang. 15. In vitro actin assembly assays and purification from Acanthamoeba. J. Bradley Zuchero. Subject Index