Synopses & Reviews
The purpose of this book is to indicate the value of HPLC in various areas of endocrinology. Each chapter provides the reader with guidelines as to how to apply HPLC in a practical fashion based on the authors' own experience of HPLC in the separation of compounds. The relevant literature is covered from 1980 onwards. Although there are a multitude of books describing applications of HPLC, this is the first volume to bring together those applications of interest to research clinicians, biochemists and other workers in the general field of endocrinology.
Synopsis
This book originates from a symposium held at the London Hospital Medical College under the auspices of Applied Chromatography Systems Ltd. to discuss the place of HPLC in the endocrinology field. Many of the authors of the present book were speakers at this symposium. It seemed to us that many endocrinolo- gists did not at that time fully appreciate the value of HPLC, and this book was designed to publicise the potential value of this technique. A survey of methods used in the steroid field Cp 185) confirmed the view that HPLC is not being used as widely, particularly in research described in clinical journals, as might be expected. We hope that this book will illustrate, albeit in a few selected areas of endocrinology, just how versatile and powerful a technique HPLC is, and en- courage those who have not yet experienced it to have a go. The beginner does not need to buy expensive instrumentation - all that is required is a pump, injector, column and detector - the rest can come later All the authors have practical experience in the use of HPLC in the particular area they discuss. All readers who discover apparent errors or who feel that the treatment of a topic of interest can be improved upon are encouraged to contact the editors. All criti- cism, especially if constructive, is welcomed. We are still learning, and other peoples' experience is always valuable.
Table of Contents
Contents: Introduction to the Theory and Practice of HPLC.- High-Performance Liquid Chromatography of Catecholamines and Their Metabolites in Biological Material.- High Performance Liquid Chromatography of the Neuropeptides: The Endogenous Opioid Peptides.- HPLC of Vitamin D and its Application to Endocrinology.- High Performance Liquid Chromatography in the Analysis of Prostaglandins, Thromboxanes and Leukotrienes.- HPLC of Gastrointestinal Hormones.- HPLC of Steroid Hormones.- The Mass Spectrometer as a Detector for HPLC: Problems of Interfacing.- Subject Index.