Synopses & Reviews
This book covers the principle hydrodynamic and calorimetric techniques for studying protein-ligand interactions. A ligand is an atom, molecule, or ion that can bind to a specific site on a protein, and the interactions between any protein and its ligands are fundamental for the protein to function properly. Written from a practical standpoint, this book is applicable to both academic and industrial scientists wishing to characterize protein-ligand systems by using a multi-disciplinary approach.
Review
"Overall I found this book to contain a great deal of useful information about many spectroscopic techniques in physical biochemistry. It would serve well as a reference book for someone who needs an introduction to the techniques."--
Biochemistry and Molecular Biology EducationReview
"Overall I found this book to contain a great deal of useful information about many spectroscopic techniques in physical biochemistry. It would serve well as a reference book for someone who needs an introduction to the techniques."--Biochemistry and Molecular Biology Education
Table of Contents
List of protocols
Abbreviations
1. Protein-ligand interactions and their analysis, Babur Z. Chowdhry and Stephen E. Harding
2. Equilibrium dialysis and rate dialysis, Bent Honoré
3. Quantitative characterization of ligand binding by chromatography, Donald J. Winzor
4. Sedimentation velocity analytical ultracentrifugation, Stephen E. Harding and Donald J. Winzor
5. Sedimentation equilibrium in the analytical ultracentrifuge, Donald J. Winzor and Stephen E. Harding
6. Surface plasmon resonance, P. Anton Van Der Merwe
7. Capillary electrophoresis, Niels H. H. Heegaard
8. Molecular electro-optics, Dietmar Porschke
9. High-flux X-ray and neutron solution scattering, Stephen J. Perkins
10. Isothermal titration calorimetry of biomolecules, Ronan O'Brien, Babur Z. Chowdhry, and John E. Ladbury
11. Differential scanning microcalorimetry, Alan Cooper, Margaret A. Nutley, and Abdul Wadood
List of suppliers
Index