Microbial Enzymes and Biotransformations

The application of recombinant DNA technology to industrial fermentation and the production of enzymes over the last 20 years has produced a host of useful chemical and biochemical substances. In Microbial Enzymes and Biotransformations, leading experts in enzyme manipulation describe in detail their cutting-edge techniques for the screening, evolution, production, immobilization, and application of enzymes. These readily reproducible methods can be used to improve enzyme function by directed evolution, to covalently immobilize enzymes, to microencapsulate enzymes and cells, and to manufacture enzymes for human health, nutrition, and environmental protection. Two overview chapters consider microorganisms as a source of metabolic and enzymatic diversity, and enzymes in the fast-moving field of enzyme biosensors. The protocols follow the successful Methods in Biotechnology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. A companion volume, Microbial Processes and Products, provides laboratory procedures for a wide range of processes and products mediated by microorganisms. Comprehensive and highly practical, Microbial Enzymes and Biotransformations offers laboratory and industrial scientists a wealth of proven enzymatic protocols that show clearly how to go from laboratory results to successful industrial applications.

The first two chapters alone are worth reading to appreciate the range of microbes and their applications in industry. Thereafter, each chapter deals with a particular topic and presents an introduction followed by an accurate set of methods, which are well set out and easy for the specialist to follow. However, this book would be a good addition to a library, although I suspect its comprehensive coverage of the subject will make it too general for personal purchase. -Robert D. Possee, CEH-Oxford "Comprehensive and practical, this book offers laboratory and industrial scientists a wealth of proven enzymatic protocols that show clearly how to go from laboratory results to successful industrial applications." - BioTech International

The development of biotechnology over the last 20 years and particularly the use of recombinant DNA techniques are expanding the opportunities for human profit of living resources. Microbial Enzymes and Biotransformation provides practical experimental laboratory procedures for a wide range of enzymes and biotransformations mediated by microorganisms. In addition, it provides a step-by-step description of the most recent developments in applied biotechnological processes useful for screening, evolution, production, immobilization, and use of enzymes.

Leading experts in enzyme manipulation describe in detail their cutting-edge techniques for the screening, evolution, production, immobilization, and application of enzymes. These readily reproducible methods can be used to improve enzyme function by directed evolution, to covalently immobilize enzymes, to microencapsulate enzymes and cells, and to manufacture enzymes for human health, nutrition, and environmental protection. Overview chapters on microorganisms as a source of metabolic and enzymatic diversity, and on the fast-moving field of enzyme biosensors are presented. Microbial Enzymes and Biotransformations offers laboratory and industrial scientists a wealth of proven enzymatic protocols that show clearly how to go from laboratory results to successful industrial applications.

Microbial Cells and Enzymes: A Century of Progress José L. Adrio and Arnold L. Demain Enzyme Biosensors Steven J. Setford and Jeffrey D. Newman Directed Evolution of Enzymes: How to Improve Nucleic Acid Polymerases for Biocatalysis Susanne Brakmann and Sascha Nico Stumpp L-Glutaminase as a Therapeutic Enzyme of Microbial Origin Abdulhameed Sabu, K. Madhavan Nampoothiri, and Ashok Pandey Enzymatic Production of D-Amino Acids Hemraj S. Nandanwar, Gurinder S. Hoondal, and Rakesh M. Vohra Screening of Microorganisms for Enzymatic Biosynthesis of Nondigestible Oligosaccharides Oskar Bañuelos, Maria del Carmen Ronchel, José Luis Adrio, and Javier Velasco Food-Grade Corynebacteria for Enzyme Production José A. Gil, Angelina Ramos, Sirin A. I. Adham, Noelia Valbuena, Michal Letek, and Luis M. Mateos Enzymatic Synthesis of (S)-Phenylalanine and Related (S)-Amino Acids by Phenylalanine Dehydrogenase Yasuhisa Asano Enzymes in Modern Detergents Susumu Ito, Tohru Kobayashi, Yuji Hatada, and Koki Horikoshi Microbial Proteases Chandran Sandhya, K. Madhavan Nampoothiri, and Ashok Pandey Proteases Produced by Halophilic Bacteria and Archaea Encarnación Mellado, Cristina Sánchez-Porro, and Antonio Ventosa Microbial Pectinases Rupinder Tewari, Ram P. Tewari, and Gurinder S. Hoondal Expression of Microbial Phytases in Yeast Systems and Characterization of the Recombinant Enzymes Xin Gen Lei and Taewan Kim Glucose Dehydrogenase for the Regeneration of NADPH and NADH Andrea Weckbecker and Werner Hummel Acetate Kinase from Methanosarcina thermophila, a Key Enzyme for Methanogenesis Prabha Iyer and James G. Ferry Immobilization of Enzymes by Covalent Attachment Scott J. Novick and J. David Rozzell Preparation of an Industrial Biocatalyst of Penicillin G Acylase on Sepabeads: Improving the Design of Penicillin G Hydrolysis Cesar Mateo, Valeria Grazu, Olga Abian, Manuel Fuentes, Gloria Fernández-Lorente, José M. Palomo, Roberto Fernández-Lafuente, and José M. Guisán Methods for Bioencapsulation of Enzymes and Cells Thomas M. S. Chang Index