Synopses & Reviews
This book describes the principles of operation of each type of microscope currently available and of use to biomedical and materials scientists.
Synopsis
Optical and electron microscopes are often used effectively despite little knowledge of the relevant theory, or even of how a particular type of microscope functions. Eventually however, proper use, interpretation of images and choices of specific applications demand an understanding of fundamental principles. This book describes the principles of operation of each type of microscope currently available and of use to biomedical and materials scientists, explains the mechanisms of image formation (contrast and its enhancement), accounts for ultimate limits on the size of observable details (resolving power and resolution) and finally provides an account of Fourier optical theory. Principles behind the photographic methods used in microscopy are described and there is some discussion of image processing methods.
Table of Contents
Preface; List of common abbreviations used; 1. Introduction; 2. Light and electrons; 3. Wave interactions; 4. Interference effects and diffraction patterns; 5. Polarized light; 6. Lenses; 7. Imaging: microscopy and diffraction; 8. Contrast; 9. Resolution; 10. The light microscope; 11. Imaging of phase objects; 12. Polarizing microscopy; 13. Prospects of biological X-ray microscopy; 14. The conventional transmission electron microscope; 15. Scanning microscopes; 16. Aspects of practical electron microscopy; 17. The quest for ultimate EM resolution; 18. Innovations in microscope development; 19. Photography; Appendix: image location; Author index; Subject index.