Table of Contents
Looking at yeast cells --Isolation and characterization of auxotropic temperature-sensitive, and UV-sensitive mutants --Meiotic mapping --Transformation of yeast --Synthetic lethal mutants --Gene replacement --Isolation of ras2 suppressors --Manipulating cell types --Isolating mutants by insertional mutagenesis --Two-hybrid protein interaction method --High-efficiency transformation of yeast --"Lazy bones" plasmid transformation of yeast colonies --Yeast DNA isolations --Yeast protein extracts --Yeast RNA isolation --Hdroxylamine mutagenesis of plasmid DNA --Assay of B-galactosidase in yeast --Plate assay for carboxypeptidase Y --Random spore analysis --Yeast vital stains --Yeast immunofluorescence --Actin staining in fixed cells --PCR protocol for PCR-mediated gene disruption --Yeast colony PCR protocol --Measuring yeast cell density by spectrophotometry --Cell synchrony --Chromatin immunoprecipitation --Flow cytometry of yeast DNA --Logarithmic growth --EMS mutagenesis --Tetrad dissection --Making a tetrad dissection needle --Picking zygotes --Determining plating efficiency --Appendices.