Synopses & Reviews
Tissue engineering and regenerative medicine represents a wide array of cell, biomaterial and cell/biomaterial based approaches focusing on the repair, augmentation, and regeneration of diseases tissues and organs. Organ Regeneration: Methods and Protocols has been assembled in response to the growing interest in organ and tissue regeneration as a means to treat disease. Topics cover methods such as isolation and characterization of cells from selected soft tissues and solid organs, preparation and evaluation of natural and synthetic biomaterial scaffolding, implantation of regenerative constructs within experimental animals, and evaluation of regenerative outcomes by molecular and histological methodologies. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Organ Regeneration: Methods and Protocols serves as a detailed guide to aid newcomers and seasoned veterans in their developmental and experimental work in tissue engineering and regenerative medicine.
Synopsis
This Methods in Molecular Biology™ book covers cell isolation and characterization, preparation of biomaterial scaffolding, implantation of regenerative constructs in experimental animals, molecular and histological methods for evaluating results and more.
Table of Contents
1. Isolation and Characterization of Human Adipose-Derived Stem Cells for Use in Tissue Engineering Benjamin M. Buehrer and Bentley and Cheatham 2. Isolation of Smooth Muscle Cells from Bladder for Generation of Engineered Urologic Organs Darell W. McCoy 3. Isolation of Urothelial Cells from Bladder Tissue Namrata Sangha 4. Isolation of Pulsatile Cell Bodies from Esophageal Tissue John W. Ludlow, Joydeep Basu, Christopher W. Genheimer, Kelly I. Guthrie, and Namrata Sangha 5. Cell Isolation Through Whole Liver Perfusion and Preparation of Hepatocytes for Cytochrome P450 Analysis Sonya O. Meheux 6. Ex Vivo Culture and Separation of Functional Renal Cells Andy Bruce, Kelly Guthrie, and Rusty Kelley 7. Isolation and Myogenic Differentiation of Mesenchymal Stem Cells (MSC) for Urologic Tissue Engineering Rongpei Wu, Guihua Liu, Shantaram Bharadwaj, and Yuanyuan Zhang 8. Xeno-Free Adaptation and Culture of Human Pluripotent Stem Cells Tori Sampsell-Barron 9. Reverse Engineering Life: Physical and Chemical Mimetics for Controlled Stem Cell Differentiation into Cardiomyocytes Gary R. Skuse and Kathleen A. Lamkin-Kennard 10. Phenotypic Analysis of Bioactive Cells for Application in Regenerative Medicine Teresa Bora Burnette and Andrew T. Bruce 11. Preparation and Evaluation of Natural Scaffold Materials for Kidney Regenerative Applications Monica A. Serban, Toyin A. Knight, and Richard G. Payne 12. Rapid Production of Autologous Fibrin Hydrogels for Cellular Encapsulation in Organ Regeneration Adelola O. Oseni, Peter E. Butler, and Alexander M. Seifalian 13. Electrospinning Tubular Scaffolds with Tissue-Like Mechanical Properties and Biomimetic Surface Features Scott Rapoport 14. Design, Fabrication, and Preparation of Synthetic Scaffolds for Urologic Tissue Engineering Richard G. Payne and Toyin A. Knight 15. Characterization of a PGA Based Scaffold for Use in a Tissue Engineered Neo-Urinary Conduit Toyin A. Knight and Richard G. Payne 16. Migration Assay to Evaluate Cellular Interactions with Biomaterials for Tissue Engineering/Regenerative Medicine Applications Kelly Guthrie, Namrata Sangha, Christopher Genheimer, Joydeep Basu, and John W. Ludlow 17. Care of Rodent Models used for Pre-Clinical Evaluation of Tissue Engineered/Regenerative Medicine Product Candidates Kim L. Mihalko 18. Bioreactor Design Considerations for Hollow Organs Jeff Fish, Craig Halberstadt, Darell McCoy, and Neil Robbins 19. Construction of a Multicoaxial Hollow Fiber Bioreactor Randall McClelland, Katherine Tech, and Jeffrey M. Macdonald 20. Microencapsulation of Pancreatic Islets for Use in a Bioartificial Pancreas Emmanuel C. Opara, John P. McQuilling, and Alan C. Farney 21. Bioengineered Skin Substitutes Pedro Lei, Hui You, and Stelios T. Andreadis 22. Formulation of Selected Renal Cells for Implantation into a Kidney Craig Halberstadt, Neil Robbins, Darell McCoy, Kelly Guthrie, Andrew Bruce, Toyin Knight, and Richard Payne 23. Human Liver Bioengineering Using a Whole Liver Decellularized Bioscaffold Pedro M. Baptista, Dipen Vyas, Emma Moran, Zhan Wang, and Shay Soker 24. A "Living Bioreactor" for the Production of Tissue Engineered Small Intestine Daniel E. Levin, Frederic G. Sala, Erik R. Barthel, Allison L. Speer, Xiaogang Hou, Yasuhiro Torashima, and Tracy C. Grikscheit 25. Tissue Engineering of Esophagus and Small Intestine in Rodent Injury Models Joydeep Basu, Kim Mihalko, Elias Rivera, Kelly I. Guthrie, Christopher Genheimer, Namrata Sangha, and John W. Ludlow 26. Scanning Electron Microscopy Evaluation of Endothelialized Tissue Engineered Constructs Sandra L. Johnson 27. Genotypic and Phenotypic Analysis of in vivo Tissue Regeneration in an Animal Model Christopher W. Genheimer 28. Histological Evaluation of Tissue Regeneration Using Biodegradable Scaffold Seeded by Autologous Cells for Tubular/Hollow Organs Applications Elias A. Rivera and Manuel J. Jayo