1. Cell Cycle Control: A System of Interlinking Oscillators
Randy Y.C. Poon
2. Substrate Phosphorylation Rates as an In Vivo Measurement of Kinase Activity
Matthew P. Swaffer
3. Real Time Monitoring of APC/C-Mediated Substrate Degradation Using Xenopus laevis Egg Extracts
Julia Kamenz, Renping Qiao, Qiong Yang, and James E. Ferrell, Jr.
4. Fluorescent Peptide Biosensors for Probing CDK Kinase Activity in Cell Extracts
Morgan Pellerano and May C. Morris
5. Phosphatase and Kinase Substrate Specificity Profiling with Pooled Synthetic Peptides and Mass Spectrometry
Andrew G. DeMarco, Pete E. Pascuzzi, W. Andy Tao, and Mark C. Hall
6. Whole-Mount Immunostaining for the Identification of Histone Modifications in the S-Phase Nuclei of Arabidopsis Roots
Hirotomo Takatsuka and Masaaki Umeda
7. Construction of a Full-Length 3'UTR Reporter System for Identification of Cell-Cycle Regulating MicroRNAs
Dominika Kazmierczak and Per Hydbring
8. Purification of Cyclin-Dependent Kinase Fusion Complexes for In Vitro Analysis
Mardo K ivom gi
9. Optimizing Cell Synchronization Using Nocodazole or Double Thymidine Block
Arif A. Surani, Sergio L. Colombo, George Barlow, Gemma A. Foulds, and Cristina Montiel-Duarte
10. Highly Synchronous Mitotic Progression in Schizosaccharomyces pombe Upon Relief of Transient Cdc2-asM17 Inhibition
Pawan Singh, Lenka Halova, and Iain Michael Hagan
11. Elucidating Human Mitosis Using an Anaphase-Like Cell-Free System
Danit Wasserman, Sapir Nachum, Meirav Noach-Hirsh, Naomi Auerbach, Evelin Sheinberger-Chorni, Taylor P Enrico, Roxane Lahmi, Michael J. Emanuele, and Amit Tzur
12. EDU (5-Ethynyl-2′-Deoxyuridine)-Coupled-Fluorescence-Intensity Analysis: Determining Absolute Parameters of the Cell Cycle
Jo o A. Ferreira, Marco Neves, Miguel Alpalh o, Pedro Pereira, Daniela Cunha, Fernando Ferreira, Ren Santus, Ana E. Sousa, and Paulo L. Filipe
13. High-Resolution Analysis of Centrosome Behavior During Mitosis
Vanessa Nunes, Margarida Dantas, Joana T. Lima, and Jorge G. Ferreira
14. Assaying Cell Cycle Progression via Flow Cytometry in CRISPR/Cas9-Treated Cells
Jonathan M. Geisinger and Tim Stearns
15. Use of Mitotic Protein Kinase Inhibitors and Phospho-Specific Antibodies to Monitor Protein Phosphorylation During the Cell Cycle
Isha Nasa, Greg B. Moorhead, and Arminja N. Kettenbach
16. Visualization of Radiation-Induced Cell Cycl