1. Production of Eukaryotic Cell-Free Lysate from Leishmania tarentolae
Wayne Johnston and Kirill Alexandrov
2. Bioinformatics Analysis and Optimization of Cell-Free Protein Expression
Alexander A. Tokmakova, Atsushi Kurotania, Mikako Shirouzu, Yasuo Fukamid, and Shigeyuki Yokoyama
3. A Cell-Free Expression Screen to Identify Fusion Tags for Improved Protein Expression
Andrew Kralicek
4. One-Pot, Microscale Cell-Free Enzyme Expression and Screening
Aarthi Chandrasekaran and Anup K. Singh
5. Cell-Free Translation of Biofuels Enzymes
Taichi E. Takasuka, Johnnie A. Walker, Lai F. Bergeman, Kirk A. Vander Meulen, Shin-ichi Makino, Nathaniel L. Elsen, and Brian G. Fox
6. Cloning-Independent Expression and Screening of Enzymes using Cell-Free Protein Synthesis Systems
Yong-Chan Kwon, Jae-Kwang Song, and Dong-Myung Kim
7. High Level Cell-Free Production of Membrane Proteins with Nanodiscs
Christian Roos, Lei Kai, Stefan Haberstock, Davide Proverbio, Umesh Ghoshdastider, Yi Ma, Slawomir Filipek, Xiaoning Wang, Volker D tsch, and Frank Bernhard
8. Cell Free Protein Based Enzyme Discovery and Protein-Ligand Interaction Study
Sabrina Guillemer, C cile Persillon, Jean-Michel Masson, and Gilles Ravot
9. Human Cell Extract-Derived Cell-Free Systems for Virus Synthesis
Tominari Kobayashi, Kodai Machida, and Hiroaki Imataka
10. Cell-Free Protein Synthesis in Microfluidic 96-Well Plates
Kirsten Jackson, Ruba Khnouf, and Z. Hugh Fan
11. Preparation of Multiple Site-Specific Mutant Proteins for NMR Studies by PCR-Directed Cell-Free Protein Synthesis
Kiyoshi Ozawa and Ruhu Qi
12. Site-Specific Incorporation of Unnatural Amino Acids into Proteins by Cell-Free Protein Synthesis
Kiyoshi Ozawa and Choy Theng Loh
13. In vitro Translation of Papillomavirus Authentic and Codon-Modified L1 Capsid Gene mRNAs in Mouse Keratinocyte Cell-Free Lysate
Kong-Nan Zhao
14. An Optimized Yeast Cell-Free Lysate System for In vitro Translation of Human Virus mRNA
Xiao Wang, Liang Zhao, and Kong-Nan Zhao
15. In vitro Translation Based Protein Kinase Substrate Identification
Szilvia K. Nagy and Tam s M sz ros
16. Preparation of Protein Arrays using Cell-Free Protein Expression
Elizabeth A. Cook and Mingyue He
17. Post-Transcriptional Control of Protein Synthesis in Drosophila S2 Cell-Free System
Motoaki Wakiyama and Shigeyuki Yokoyama
18. Cell-Free Membrane Protein Expression
Tomomi Kimura-Soyema, Mikako Shirouzu, and Shigeyuki Yokoyama
19. The PURE System for Protein Production
Yoshihiro Shimizu, Yutetsu Kuruma, Takashi Kanamori, and Takuya Ueda
20. A Cell-Free Protein Synthesis System from Insect Cells
Toru Ezure, Takashi Suzuki, and Eiji Ando
21. A Cell-Free Expression Platform for Production of Protein Microarrays
Xristo Z rate and David W. Galbraith