Synopses & Reviews
This concise, truncated version of Nagy, Varghese and Agarwal's
Practical Manual of In Vitro Fertilization is comprised of select clinical chapters for a portable, affordable and up-to-date resource.
Clinical Embryology covers a variety of topics, including:
- Sperm processing methods
- Embryo culture methods
- Insemination procedures
- Embryo evaluation
- Genetic testing of embryos
- Cryopreservation
- Embryo transfer
Also included are two new chapters on predicting embryo viability and implantation potential
About the Author
Zsolt Peter Nagy, MD, PhD, HCLD (ABB), EMB (ACE), Scientific and Laboratory Director, Reproductive Biology Associates, Atlanta, GA, USA
Alex C. Varghese, PhD, Senior Embryologist, Montreal Reproductive Centre, Montreal, QC, Canada
Ashok Agarwal, PhD, HCLD (ABB), EMB (ACE), Director, Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA
Table of Contents
Journey of Human Gametes In Vitro: 1978 to 2010.- Part I: Sperm Processing Methods.- Sperm Processing for IVF.- PESA/TESA/TESE Sperm Processing.- Processing Sperm Samples in HIV-Positive Patients.- Part II: Embryo Culture Methods.- Culture Media in IVF: Decisions for the Laboratory.- Short Culture: Day 1/Day 2/Day 3 Embryo Culture.- Extended Culture in IVF.- In Vitro Maturation of Human Oocytes.- In Vivo Embryo Culture Device.- Microfluidics for Gamete Manipulation and Embryo Culture.- Part III: Insemination Procedures.- Intrauterine Insemination.- Conventional IVF Insemination.- Oocyte Denuding.- Intracytoplasmic Sperm Injection.- Part IV: Embryo Evaluation and Grading.- Pronuclear Scoring in Human In Vitro Fertilization.- Cumulative Morphological Assessment of Embryo Quality.- Metablomics: The
ViaMetrics-E™ Procedure for Assessing Embryo Viability.- Oxygen Consumption as an Indicator of Oocyte and Embryo Viability.- Gene Expression Changes During Human Early Embryo Development: New Applications for Embryo Selection.- Amino Acid Turnover as a Biomarker of Embryo Viability.- Real-Time Embryo Monitoring Devices for Embryo Selection.- Predicting Embryo Implantation Potential and Viability Using Automated Time Lapse Analysis (Eeva).- Using Dark Field Embryo Imaging to Assess Embryo Development Potential and Viability.- Part V: Genetic Testing of Embryos.- Embryo Biopsy for PGD: Current Perspective.- Microarrays and CGH for PGD of Chromosomal Abnormalities and Gene Defects.- Part VI: Cryopreservation.- Sperm Cryopreservation.- Slow Freezing of Oocytes.- Vitrification: Research in Animal Models.- Oocyte Vitrification.- Slow Freezing of Embryos.- Vitrification of Embryos.- Directional Freezing of Reproduction Cells, Tissues, and Organs.- Ovarian Tissue Cryopreservation.- Part VII: Embryo Transfer.- Assessment of Uterine Receptivity.- Single Embryo Transfer.- Ultrasound-Guided Embryo Transfer (Abdominal/Vaginal): An Evidence-Based Evaluation.