Synopses & Reviews
DNA Microarrays: A Practical Approach is the first comprehensive overview of an exciting and powerful new technology. DNA microarrays, or biochips, are small glass chips embedded with ordered rows of DNA, providing a massive parallel platform for data gathering and representing a fundamental technical advance in biomedical research. Such biochips gather data at an unprecedented rate by enabling the use of advanced fabrication, detection, and data mining technologies. Written and edited by experts in the field, this book provides fascinating insight into this remarkable advancement. It opens with an introduction to the technology of DNA microarrays, emphasizing the methodological fundamentals of biochips, and continues with descriptions of the use of confocal scanning in microarray detection and techniques for the efficient cloning and screening of differentially expressed genes. The chapters address many topics, among them assay optimization, antisense scanning arrays, the manufacture of molecular arrays, and gene expression analysis. Also addressed are the uses of expression data in bioinformatics, of active microelectronic arrays for DNA hybridization analysis, and of microarray technology in pharmacogenomics. DNA Microarrays: A Practical Approach is ideal for researchers investigating patterns of gene expression (and the relationship with disease), and it is essential for any researcher interested in the use of biochips.
Review
"DNA Microarrays (biochips) are small glass chips embedded with ordered rows of DNA which allow data to be gathered quickly. This volume provides a guide to this new technology, addressing the use of confocal scanning in microarray detection, techniques for cloning and screening of differentially expressed genes, the optimization of enzymatic assays using oligonucleotide arrays, antisense oligonucleotide scanning arrays, the production of molecular arrays using ink-jet printing technology, gene expression analysis via cDNA microarrays, the use of expression data in bioinformatics, active microelectronic arrays for DNA hybridization analysis, and the applications of microarray technology in pharmacogenomics. In each article, methodological details and current applications are described. Contributors primarily consist of biochemists and researchers in pharmaceutical companies in the US, Switzerland, and the UK."--SciTech Book News
Synopsis
Written and edited by experts in the field, this book provides valuable insight into the remarkable advancement of DNA microarrays. It is essential for researchers investigating patterns of gene expression and useful for all those interested in the use of biochips.
Table of Contents
List of Contributors
Abbreviations
1. Genes, genomes, and chips, Mark Schena and Ronald W. Davis
2. Confocal scanning microscopy in microarray detection, Mack J. Schermer
3. Representational differences analysis and microarray hybridization for efficient cloning and screening of differentially expressed genes, Stanley F. Nelson and Christopher T. Denny
4. Use of oligonucleotide arrays in enzymatic assays: assay optimization, Stephen Case-Green, Clare Pritchard, and Edwin M. Southern
5. Antisense oligonucleotide scanning arrays, John K. Elder et al.
6. Application of ink-jet printing technology to the manufacture of molecular arrays, Thomas P. Theriault, Scott C. Winder, and Ronald C. Gamble
7. Gene expression analysis via cDNA microarrays of laser capture microdissected cells from fixed tissue, Ranelle C. Salunga et al.
8. Expression data and the bioinformatics challenges, Joel Lloyd Bellenson
9. Active microelectronic arrays for DNA hybridization analysis, Michael J. Heller et al.
10. Gene chips and microarrays: applications in disease profiles, drug target discovery, drug action and toxicity
Appendix
Index