Synopses & Reviews
This convenient laboratory manual covers the theory and application of all the commonly used FISH procedures for both the research and clinical service laboratory. Readers are provided with the theoretical principles which underlie each procedure, and are then guided through the protocols in a systematic, easy to follow manner. Because FISH procedures are particularly sensitive to background and subtle variations in sample quality, the trouble-shooting sections are invaluable to the beginner interested in developing FISH capabilities in their laboratory. Areas covered include probe production and labeling; FISH gene mapping; murine, fiber, and RNA FISH; CGH and microchips; 3D FISH; SKY and M-FISH; FISH and microarrays; and FISH in clinical cytogenetics.
Review
"The book will be of value to researchers and students in molecular pathology, cancer biology, and genomics."--SciTech Book News
Table of Contents
List of protocols
Abbreviations
1. Introduction, Sabine Mai, Barbara G. Beatty, and Jeremy A. Squire
2. FISH probes and labelling techniques, Patricia Bray-Ward
3. Human chromosome mappin of single copy genes, Barbara G. Beatty and Stephen W. Scherer
4. Murine chromosome preparation, Sabine Mai and Francis Wiener
5. High resolution FISH mapping using chromatin and DNA fibre, Henry H. Q. Heng
6. Applications of RNA FISH for visualizing gene expression and nuclear architecture, Rose Tam et al.
7. FISH on three-dimensionally preserved nuclei, I. Solovei et al.
8. Comparative genomic hybridization on metaphase chromosomes and DNA chips, Stefan Joos, Carsten Schwänen, and Peter Lichter
9. FISH in clinical cytogenetics, Jeremy A. Squire, P. Marrano, and E. Kolomietz
10. Multicolour FISH and spectral karyotyping, Jane Bayani and Jeremy A. Squire
11. cDNA microarrays for fluorescent hybridization analysis of gene expression, Javed Khan et al.