Synopses & Reviews
Reflecting the expanding field's need for reliable protocols, Fluorescence Spectroscopy and Microscopy: Methods and Protocols offers techniques from a worldwide team of experts on this versatile and vital subject.
Table of Contents
Part I: Steady-State Fluorescence Spectroscopy (SSFS) 1. How to Collect National Institute of Standards and Technology (NIST) Traceable Fluorescence Excitation and Emission Spectra Adam Matthew Gilmore 2. Steady State Fluorescence Polarization/Anisotropy for the Study of Protein Interactions Nicholas G. James and David M. Jameson 3. Quantitative Fluorescence Spectral Analysis of Protein Denaturation Ivo H.M. van Stokkum and Sergey P. Laptenok 4. High-Pressure Fluorescence Applications Mariano Dellarole and Catherine A. Royer Part II: Time-Resolved Fluorescence Spectroscopy (TRFS) 5. Frequency Domain Fluorometry: Theory and Application Carissa M. Vetromile and David M. Jameson 6. Polar Plot Representation of Time-Resolved Fluorescence John Paul Eichorts, Kai wen Teng, and Robert M. Clegg 7. Ensemble and Single Molecule Detected Time Resolved FRET Methods in Studies of Protein Conformations and Dynamics Tomer Orevi, Eitan Lerner, Gil Rahamim, Dan Amir, and Elisha Haas 8. MD + QM Correlations with Tryptophan Fluorescence Spectral Shifts and Lifetimes Patrik R. Callis and Jose R. Tusell 9. Analysis of Time-Dependent Red Shifts in Fluorescence Emission from Tryptophan Residues in Proteins Dmitri Toptygin 10. Global Analysis of Time-Resolved Fluorescence Data Anatoli V. Digris, Eugene G. Novikov, Victor V. Skakun, and Vladimir V. Apanasovich 11. Nanometrology David J.S. Birch and Philip Yip 12. Upconversion Spectrophotofluorometry Arianna Biesso, Jianhua Xu, and Jay R. Knutson 13. Subpicosecond Kerr-Gate Spectrofluorometry Sergey P. Laptenok, Patrick Nürnberger, Andras Lukacs, and Marten H. Vos 14. Photo-Induced Electron Transfer Modeling to Simulate Flavoprotein Fluorescence Decay Nadtanet Nunthaboot, Kiattisak Lugsanangarm, Arthit Nueangaudom, Somsak Pianwanit, Sirirat Kokpol, and Fumio Tanaka Part III: Fluorescent Probe Development (FPD) 15. Biosynthetic Incorporation of Trp Analogs in Proteins Jaap Broos 16. Optimization of Fluorescent Proteins Daphne S. Bindels, Joachim Goedhart, Mark A. Hink, Laura van Weeren, Linda Joosen, and Theodorus W.J. Gadella, Jr. 17. Monitoring Membrane Properties and Apoptosis Using Membrane Probes of the 3-Hydroxyflavone Family Zeinab Darwich, Andrey S. Klymchenko, and Yves Mély Part IV: Fluorescence Microscopy: Fluorescence Recovery After Photobleaching (FRAP) 18. Rectangle FRAP for Measuring Diffusion with a Laser Scanning Microscope Ranhua Xiong, Hendrik Deschout, Jo Demeester, Stefaan C. De Smedt, and Kevin Braeckmans Part V: Fluorescence Microscopy: Förster Resonance Energy Transfer Imaging (FRETim) 19. A Quantitative Protocol for Intensity-Based Live Cell FRET Imaging Clemens F. Kaminski, Eric. J. Rees, and Gabriele S. Kaminski Schierle Part VI: Fluorescence Microscopy: Fluorescence Lifetime Imaging (FLIM) 20. Widefield Fluorescence Lifetime Imaging with Multi-Anode Detectors Roland Hartig, Yury Prokazov, Evgeny Turbin, and Werner Zuschratter 21. Global Analysis of FRET- FLIM Data in Live Plant Cells Sergey P. Laptenok, Joris J. Snellenburg, Christoph A. Bücherl, Kai R. Konrad, and Jan Willem Borst 22. Time-Resolved Fluorescence Anisotropy Imaging Klaus Suhling, James Levitt, and Pei-Hua Chung 23. Multi-Modal Fluorescence Imaging Spectroscopy Martijn H.W. Stopel, Christian Blum, and Vinod Subramaniam Part VII: Fluorescence Microscopy: Fluorescence Fluctuation Spectroscopy (FFS) 24. Application of Fluorescence Correlation Spectroscopy (FCS) to Measure the Dynamics of Fluorescent Proteins in Living Cells Thomas Weidemann 25. Fluorescence Cross-Correlation Spectroscopy (FCCS) in Living Cells Xiaoxiao Ma, Yong Hwee Foo, and Thorsten Wohland 26. Quantifying Lipid-Protein Interaction by Fluorescence Correlation Spectroscopy (FCS) Ana M. Melo, Manuel Prieto, and Ana Coutinho 27. PET-FCS: Probing Rapid Structural Fluctuations of Proteins and Nucleic Acids by Single-Molecule Fluorescence Quenching Markus Sauer and Hannes Neuweiler 28. Z-Scan Fluorescence Correlation Spectroscopy as a Tool for Diffusion Measurements in Planar Lipid Membranes Tomáš Steinberger, Radek Macháň, and Martin Hof 29. Scanning Fluorescence Correlation Spectroscopy (SFCS) with a Scan Path Perpendicular to the Membrane Plane Paul Müller, Petra Schwille, and Thomas Weidemann 30. Implementation and Application of Pulsed Interleaved Excitation for Dual-Color FCS and RICS Jelle Hendrix and Don C. Lamb 31. Quantitative Study of Protein-Protein Interactions in Live Cell by Dual Color Fluorescence Lifetime Cross-Correlation Spectroscopy Sergi Padilla-Parra, Nicolas Audugé, Maïté Coppey-Moisan, and Marc Tramier 32. Brightness Experiments Patrick J. Macdonald, Jolene Johnson, Yan Chen, and Joachim D. Mueller 33. Global Analysis of Autocorrelation Functions and Photon Counting Distributions in Fluorescence Fluctuation Spectroscopy Victor V. Skakun, Anatoli V. Digris, and Vladimir V. Apanasovich 34. Simulation of Autocorrelation Function and Photon Counting Distribution in Fluorescence Fluctuation Spectroscopy Igor P. Shingaryov, Victor V. Skakun, and Vladimir V. Apanasovich Part VIII: Fluorescence Microscopy: Single-Molecule Fluorescence Spectroscopy (smFS) 35. Single-Molecule Fluorescence of Nucleic Acids Kaley McCluskey, Euan Shaw, Daniel A. Lafontaine, and J. Carlos Penedo 36. Photoswitchable Fluorescent Proteins for Superresolution Fluorescence Microscopy Above the Diffraction Limit of Light Susana Rocha, Herlinde De Keersmaecker, Hiroshi Uji-i, Johan Hofkens, and Hideaki Mizuno