Synopses & Reviews
In recent years there has been a growing demand to achieve more rapid and accurate detection of RONs (reactive oxygen and nitrogen species), which affect normal physiological processes and pathological conditions, and also of redox stress. In Free Radicals and Antioxidant Protocols, Second Edition, world-renowned researchers respond to this need, considering the most recent analytical and technological aspects of the detection and determination of RONs, and of oxidative, nitrative, nitrosative, and redox stress in biological systems in vivo and in vitro. Chapters include protocols to establish biomarkers for the oxidative stress-induced genetic damage in cellular and animal models, provide novel methods and technologies for analyzing peroxidized lipids, describe techniques for gene therapy, and present methods of EPR imaging of free radicals and oxygen in vivo. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and user-friendly, Free Radicals and Antioxidant Protocols, Second Edition, is an essential guide for the novice and expert researcher alike.
Review
From the reviews of the second edition: "The book is organized into four sections which draw on contributions from several experts in the field of oxidative stress. ... Based on a collection of articles in the four sections, the book is a good reference for those interested in free radical science including new methodologies to measure both oxidative stress and antioxidants using in vivo and in vitro models." (Silvia Berlanga de Moraes Barros, Brazilian Journal of Pharmaceutical Sciences, May, 2010)
Synopsis
This cutting-edge and updated book offers methods for the rapid detection of RONs and redox stress. It includes in-depth analysis of natural and synthetic antioxidants, and also of DNA oxidation, oxidative lipidomics, and biomarkers.
Table of Contents
Contents Preface Contributors Part I Reactive Oxygen and Nitrogen Species 1. In Vivo Imaging of Free Radicals and Oxygen Deepti S. Vikram, Brian K. Rivera, and Periannan Kuppusamy 2 . In Vivo Measurement of Tissue Oxygen using Electron Paramagnetic Resonance Spectroscopy with Oxygen-sensitive Paramagnetic Particle, Lithium Phthalocyanine F. Hyodo, S. Matsumoto, E. Hyodo, A. Matsumoto, K. Matsumoto, and M. C. Krishna 3. Measurement of Plasma Nitrite by Chemiluminescence Enika Nagababu and Joseph M Rifkind 4. Determination of Glutathione, Mitochondrial Transmembrane Potential, and Cytotoxicity in H9c2 Cardiomyoblasts Exposed to Reactive Oxygen and Nitrogen Species K. Sathishkumar, Xueli Gao, Achuthan C. Raghavamenon, Narasimham L. Parinandi, and Rao M. Uppu Part II Natural and Synthetic Antioxidants 5. Phenolic Acids and Flavonoids: Ocurrence and Analytical Methods Constantine D. Stalikas 6. Design, Synthesis, and Action of Antiatherogenic Antioxidants Osamu Cynshi, Kunio Tamura, and Etsuo Niki 7. Preparation of Drug-Loaded Polymeric Nanoparticles and Evaluation of the Antioxidant Activity against Lipid Peroxidation Adriana R. Pohlmann, Scheila Rezende Schaffazick, Tânia B. Creczynski-Pasa, and Sílvia S. Guterres 8. Nanoparticle and Iron Chelators as a Potential Novel Alzheimer Therapy Gang Liu, Ping Men, George Perry, and Mark A. Smith 9. Synthesis and Characterization of Polymer Nanocarriers for the Targeted Delivery of Therapeutic Enzymes Eric Simone, Thomas Dziubla , Vladimir Shuvaev, and Vladimir R. Muzykantov 10. Assessment of Antioxidant Activity of Eugenol In Vitro and In Vivo Enika Nagababu, Joseph M. Rifkind, Sesikeran Boindala, and Lakshmaiah Nakka Part III Cellular Oxidative Stress 11. The Generation of Stable Oxidative Stress-Resistant Phenotypes in Chinese Hamster Fibroblasts Chronically Exposed to Hydrogen Peroxide or Hyperoxia Douglas R. Spitz and Shannon J. Sullivan 12. A Simple Method for Effective and Safe Removal of Membrane Cholesterol from Lipid Rafts in Vascular Endothelial Cells: Implications in Oxidant-Mediated Lipid Signaling Michelle A. Kline, E. S. O'Connor Butler, Adam Hinzey, Sean Sliman, Clay B. Marsh, Rao M. Uppu, and Narasimham L. Parinandi 13. Superoxide Dismutase - A Target for Gene Therapeutic Approach to Reduce Oxidative Stress in Erectile Dysfunction W. Deng , T.J. Bivalacqua , W. Hellstrom , S. N. Murthy , and Philip J. Kadowitz 14. Assessing the Reductive Capacity of Cells by Measuring the Recycling of Ascorbic and Lipoic Acids James M. May 15. Biomarkers of Oxidative Stress: Methods and Measures of Oxidative DNA Damage (COMET Assay) and Telomere Shortening Muthuswamy Balasubramanyam, Antonysunil Adaikalakoteswari, Zaheer Sameermahmood, and Viswanathan Mohan 16. Simultaneous Analysis of Mutiple Redox-Sensitive and Apoptotic Gene Expression in Hypothalamic Neurons Exposed to Cholesterol Ozonation Products K.Sathishkumar, Achuthan C. Raghavamenon, Karunakaran Ganeshkumar, Rameshwari Telaprolu and Rao M. Uppu 17. Redox Homeostasis and Cellular Stress Response in Aging and Neurodegeneration Vittorio Calabrese, Cesare Mancuso, Riccardo Lentile, A.M. Giuffrida Stella, and D. Allan Butterfield. 18. Gene Therapy Techniques for the Delivery of Endothelial Nitric Oxide Synthase to the Lung for Pulmonary Hypertension W. Deng, T.J. Bivalacqua, W. Hellstrom, Subramanyam N. Murthy, and Philip J. Kadowitz Part IV DNA Oxidation, Oxidative Lipidomics, and Biomarkers 19. A General Method for Quantifying Sequence Effects on Nucleobase Oxidation in DNA Yelena Margolin and Peter C. Dedon 20. Analysis of Urinary 8-Oxo-7,8-dihydro-2'-deoxyguanosine by Liquid Chromatography-Tandem Mass Spectrometry Mark D. Evans, Rajinder Singh, Vilas Mistry, Peter B. Farmer, and Marcus S. Cooke 21. Oxidative Lipidomics of Apoptosis: Quantitative Assesment of Phospholipid Hydroperoxides in Cells and Tissues Vladimir A. Tyurin, Yulia Y. Tyurina, Vladimir B. Ritov, Andriy Lysytsya, Andrew A. Amoscato, Patrick M. Kochanek, Ronald Hamilton, Steven T. DeKosky, Joel S. Greenberger, Hülya Bayir, and Valerian E. Kagan 22. Simultaneous Analysis of Multiple Lipid Oxidation Products in vivo by Liquid Chromatographic-Mass Spectrometry (LC-MS) Huiyong Yin, Todd Davis, and Ned A. Porter 23. Lipoxygenase-Catalyzed Phospholipid Peroxidation: Preparation, Purification, and Characterization of Phosphatidylinositol Peroxides E. Susan O'Connor Butler, Jessica N. Mazerik, Jason P. Cruff, Shariq I. Sherwani, Barbara K. Weis, Clay B. Marsh, Achuthan C. Raghavamenon, Rao M. Uppu, Harald H.O. Schmid, and Narasimham L. Parinandi 24. Oxidized Low Density Lipoprotein Sampath Parthasarathy, Achuthan C. Raghavamenon, Mahdi Omar Garelnabi, and Nalini Santanam 25. Detection and Localization of Markers of Oxidative Stress by In Situ Methods: Application in the Study of Alzheimer Disease Paula L. Moreira, Lawrence M. Sayre, Xiongwei Zhu, Akihiko Nunomura, Mark A. Smith, and George Perry 26. Enzyme Immunoassay of Isoprostanes Denis M. Callewaert and Charles Sloan 27. Application of Membrane Extraction with Sorbent Interface for Breath Analysis Victor Ma, Heather Lord, Melissa Morley, and Janusz Pawliszyn