Synopses & Reviews
Synopsis
1. In Vitro Assembly of Nucleosomes For Binding/Remodeling Assays
Arnob Dutta and Jerry L. Workman
2. An Assay for Measuring Histone Variant Exchange within Nucleosomes In Vitro
Liette Laflamme, Benoit Guillemette, and Luc Gaudreau
3. Purification of Yeast Native Reagents for the Analysis of Chromatin Function-I: Nucleosomes for Reconstitution and Manipulation of Histone Marks
Nicolas Lacoste, Wajid Bhat, and Jacques C t
4. Purification of Yeast Native Reagents for the Analysis of Chromatin Function-II: Multiprotein Complexes and Biochemical Assays
Nicolas Lacoste, Wajid Bhat, and Jacques C t
5. Histone Purification from Saccharomyces Cerevisiae
Frederic Jourquin and Vincent G li
6. Analytical Ultracentrifuge Analysis of Nucleosomes Assembled from Recombinant, Acid Extracted, HPLC-Purified Histones
Manjinder S. Cheema and Juan Ausi
7. Silac-Based Quantitative Strategies for Accurate Histone Post-Translational Modifications Profiling Across Multiple Biological Samples
Alessandro Cuomo, Monica Soldi, and Tiziana Bonaldi
8. Characterization of Individual Histone Post-Translational Modifications and their Combinatorial Patterns by Mass Spectrometry-Based Proteomics Strategies
Simone Sidoli and Benjamin A. Garcia
9. Production and Purification of Antibodies against Histone Modifications
Benoit Guillemette, Ian Hammond-Martel, Hugo Wurtele, and Alain Verreault
10. Immunofluorescence of Histone Proteins
Hilmar Strickfaden and Michael J. Hendzel
11. Acid-Urea Gel Electrophoresis and Western Blotting Of Histones
Catherine A. Hazzalin and Louis C. Mahadevan
12. Chromatin Immunoprecipitation of Histone Modifications in Fission Yeast
Jean Mbogning and Jason C. Tanny
13. A Spiking Strategy for Chip-Chip Data Normalization in S. Cerevisiae
C lia Jeronimo and Fran ois Robert
14. High-Resolution Genome-Wide Mapping Of Nucleosome Positioning And Occupancy Level Using Paired-End Sequencing Technology
Myl ne Brunelle, S bastien Rodrigue, Pierre- tienne Jacques, and Nicolas G vry
15. Physarum Polycephalum for Studying the Function of Histone Modifications In Vivo
Vanessa Menil-Philippot and Christophe Thiriet
16. A Method for Large Scale Screening of Random Sequence Libraries to Determine the Function of Unstructured Regions from Essential Proteins
Jean-Fran ois Millau, Benoit Guillemette, and Luc Gaudreau
Synopsis
This volume provides a comprehensive list of protocols for molecular biologists, biochemists and geneticists. Chapters cover protocols that further the study into protein complexes that modify chromatin either by adding or removing post-translational modifications, or by exchanging histone variants within the nucleosome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Histones: Methods and Protocols aims to ensure successful results in the further study of this vital field.