Synopses & Reviews
Synopsis
This book provides a comprehensive description and detailed summary of the genetics, structure, function, mechanisms of action, evolution and engineering of homing endonucleases and inteins.
Table of Contents
Back to Basics: Structure, Function, Evolution and Application of Homing Endonucleases and Inteins.- Homing Endonucleases: A Historic Perspective.- The LAGLIDADG Homing Endonuclease Family.- HNH Endonucleases.- GIY-YIG Homing Endonucleases - Beads on a String.- His-Cys Homing Endonucleases.- Group I Introns and Their Maturases: Un-invited, but Welcome Guests.- Group II Intron Homing Endonucleases; Ribonucleoprotein Complexes With Programmable Target Specificity.- Free-Standing Endonucleases of T-Even Phage: Freeloaders or Functionaries?- Function and Evolution of HO and VDE Endonucleases in Fungi.- Engineering Homing Endonucleases for Genomic Applications.- Inteins - A Historic Perspective.- Origins and Evolution of Inteins and Other Hint Domains.- Biochemical Mechanisms of Intein-Mediated Protein Splicing.- The Structure and Function of Intein-Associated Homing Endonucleases.- Harnessing Inteins for Protein Purification and Characterization.- Production of Cyclic Proteins and Peptides.- Inteins for Split-Protein Reconstitutions and Their Applications.- Intein Reporter and Selection Systems.- Industrial Applications of Intein Technology.