Synopses & Reviews
In recent years, the advancing science and increasing availability of assisted reproduction have given new hope to infertile couples. However, the use of IVF and ART has also led to marked increases in the number of multiple-infant live births. This poses a public health concern, as these neonates have a higher rate of pre-term delivery, compromising their survival chances and increasing their risk of lifelong disability. By optimizing the selection of gametes and embryos with high probabilities of implantation, it is possible to reduce the number of embryos transferred and, by extension, the number of high-risk multiple gestations, while maintaining or increasing pregnancy rates.
Human Gametes and Preimplantation Embryos: Assessment and Diagnosis provides a broad yet concise overview of established and developing methodologies for assessment of gamete and embryo viability in assisted reproduction. This book elucidates the best practices for precisely selecting viable specimens based on morphology and cleavage rate and covers the spectrum of emerging adjunctive technologies for predicting reproductive potential. The authors present their extensive knowledge of "omics" approaches (genomics, transcriptomics, proteomics, and metabolomics), with unbiased delineation of the associated advantages and potential pitfalls. This valuable clinical resource is well suited to infertility specialists, Ob/Gyn physicians, IVF laboratory technicians, and researchers in the fields of embryology and reproductive medicine.
Synopsis
In recent years, the advancing science and increasing availability of assisted reproduction have given new hope to infertile couples. However, the use of IVF and ART has also led to marked increases in the number of multiple-infant live births. This poses a public health concern, as these neonates have a higher rate of pre-term delivery, compromising their survival chances and increasing their risk of lifelong disability. By optimizing the selection of gametes and embryos with high probabilities of implantation, it is possible to reduce the number of embryos transferred and, by extension, the number of high-risk multiple gestations, while maintaining or increasing pregnancy rates. Human Gametes and Preimplantation Embryos: Assessment and Diagnosis provides a broad yet concise overview of established and developing methodologies for assessment of gamete and embryo viability in assisted reproduction. This book elucidates the best practices for precisely selecting viable specimens based on morphology and cleavage rate and covers the spectrum of emerging adjunctive technologies for predicting reproductive potential. The authors present their extensive knowledge of "omics" approaches (genomics, transcriptomics, proteomics, and metabolomics), with unbiased delineation of the associated advantages and potential pitfalls. This valuable clinical resource is well suited to infertility specialists, Ob/Gyn physicians, IVF laboratory technicians, and researchers in the fields of embryology and reproductive medicine.
Synopsis
This book surveys new and established methods for assessing gamete and embryo viability in assisted reproduction, covering best practices for selecting viable specimens and the spectrum of emerging adjunctive technologies for predicting reproductive potential.
Table of Contents
1. Current Practices and Regulations for Embryo Transfer Worldwide: Implications on IVF Outcome Baris Ata and Emre Seli 2. Analysis of the Zona Pellucida as an Indicator of Oocyte Developmental Potential Markus Montag, Thomas Schimming, and Maria Köster 3. Morphological Assessment of Oocytes, Pronuclear and Cleavage Stage Embryos Laura Rienzi, Benedetta Iussig, and Filippo Ubaldi 4. Morphological Assessment of Blastocyst Stage Embryos: Types of Grading Systems and Their Reported Outcomes Basak Balaban and David K. Gardner 5. Real Time Imaging Strategies to Improve Morphological Assessment María Cruz, Manuel Muñoz, and Marcos Meseguer 6. Limitations and Benefits of Morphologic Embryo Assessment Strategies: How Far Can Morphological Assessment Go in the Identification of Viable Embryos? Denny Sakkas and David K. Gardner 7. Sperm Morphologic Characteristics and Their Impact on Embryo Quality and Pregnancy Outcome Bhushan K. Gangrade and Ashok Agarwal 8. Objective Biomarkers of Sperm Development and Fertility: Assessment of Sperm-Zona Pellucida Binding Ability and Hyaluronic Acid Mediated Selection of Sperm for ICSI Fertilization Gabor Huszar 9. Selecting the Best Sperm and Its Implications in Clinical Practice Juan G. Alvarez 10. The Origins of Aneuploidy in Human Embryos Elpida Fragouli and Joy Delhanty 11. Preimplantation Genetic Screening and Diagnosis Using Fluorescent In Situ Hybridization (FISH) Tsilya Gerasimova and Maria D. Lalioti 12. Single Nucleotide Polymorphisms and Next Generation Sequencing Alan H. Handyside and Dagan Wells 13. Use of Comparative Genomic Hybridization (CGH) and Microarray-CGH for Preimplantation Genetic Screening Leeanda Wilton and Dagan Wells 14. Quantitative SNP Array and Real-Time PCR Based Human Preimplantation Embryo Aneuploidy Screening Nathan R. Treff 15. Regulation of Gene Expression in the Oocyte and Early Embryo: Implications for Transcriptomic and Proteomic Embryo Assessment Helena Kristiansson and Emre Seli 16. Transcriptomics Technology: Promise and Potential Pitfalls Can Bruce and Asli Uyar 17. Transcriptomic Analysis of Cumulus and Granulosa Cells as a Marker of Embryo Viability Elpida Fragouli