Synopses & Reviews
Synopsis
Explore the mechanisms of life on a sub-cellular scale
Laboratory Investigations in Cell and Molecular Biology features 21 introductory lab procedures for undergraduate biology majors. Designed to be completed with minimal equipment and preparation time, these labs are suitable for demonstrations as well as laboratory coursework. Simple biochemistry protocols give way to more focused organelle study, and eventually to more advanced molecular biology procedures including restriction mapping techniques. Students learn basic assays, data analysis, and good laboratory practices while gaining a deeper understanding of life at the molecular level.
Table of Contents
Preface.
Introductory Chapter. Writing a Laboratory Report.
Project 1. Microscopy.
Project 2. Cellular Carbohydrates.
Project 3. Cellular Nucleic Acids.
Project 4. Staining of Chromodomal DNA.
Project 5. Spectrophotometry of DNA and RNA.
Project 6. Electrophoresis of Hemoglobin.
Project 7. Electrophoresis of Serum Proteins.
Project 8. Isozyme Patterns of Lactate Dehydrogenase.
Project 9. Membrane Permeability.
Project 10. Cell Fractionation.
Project 11. Succinate Dehydrogenase Activity of Mitochondria.
Project 12. Chromatography of Photosynthetic Pigments.
Project 13. The Hill Reaction in Isolated Chloroplasts.
Project 14. Cell Motility.
Project 15. Flagellar Regeneration in Chlamydomonas.
Project 16. Spermatogenesis in the Grasshopper.
Project 17. Restriction Mapping of Phage Lambda DNA.
Project 18. Highly Repetitive DNA in the Bovine Genome.
Project 19. The Human Karyotype.
Project 20. C-Banding of Human Chromosomes.
Project 21. DNA Replication.
Project 22. Transcription of Ribosomal RNA.
Project 23. Photomicrography.
Instructor's Guide.