Synopses & Reviews
All experiments are correlated to Nester's Microbiology: A Human Perspective, 6/e, but can be used with any non-majors/allied health microbiology textbook.
Table of Contents
Part 1 Basic Microbiology Exercise 1 Ubiquity of Microorganisms Exercise 2 Pure Culture and Aseptic Technique Exercise 3 Introduction to the Compound Light Microscope Exercise 4 The Use of the Immersion Lens and Determining Motility in a Wet Mount Exercise 5 Simple Stains: Positive and Negative Stains Exercise 6 Differential and Special Stains Exercise 7 Chemically Defined, Complex, Selective, and Differential Media Exercise 8 Quantification of Microorganisms Exercise 9 Aerobic and Anaerobic Growth Exercise 10 The Effect of Incubation Temperature on Generation Time Exercise 11 Moist and Dry Heat Sterilization: Thermal Death Point and Thermal Death Time Exercise 12 Control of Microbial Growth with Ultraviolet Light Exercise 13 Osmotic Pressure and Its Effect on the Rate and Amount of Microbial Growth Exercise 14 Antiseptics and Antibiotics Exercise 15 Selection of Bacterial Mutants Resistant to Antibiotics Exercise 16 Transformation: A Form of Genetic Recombination Exercise 17 Bacterial Conjugation Exercise 18 Gene Regulation: Induction and Catabolite Repression Part 2 The Other Microbial World Exercise 19 Microscopic Identification of Fungi Exercise 20 Paratitology: Protozoa and Helminths Exercise 21 Titering Prokaryotic Viruses Part 3 Microbes and Humans Exercise 22 Normal Skin Flora Exercise 23 Respiratory Microorganisms Exercise 24 Identification of Enteric Gram-Negative Rods Exercise 25 Clinical Unknown Identification Part 4 Immunology Exercise 26 Differential White Blood Cell Stains Exercise 27 Lysosome, an Enzymatic Form of Natural Resistance Exercise 28 Lancefield Grouping of Pathogenic Streptococci Exercise 29 Use of an Enzyme-Linked Immunoabsorbent Assay (ELISA) Test of Coccidiodes immitis Identification Exercise 30 An Ouchterlony Double Immunodiffusion Test for Coccidiodes immitis Identification Part 5 Public Health Exercise 31 What is the Role of Propionibacterium acnes in Acne? Exercise 32 Bacteriological Examination of Water: Multiple-Tube Fermentation and Membrane Filter Techniques Part 6 Applications of Biotechnology Exercise 33 Identifying DNA with Restriction Enzymes Exercise 34 Identification of Bacteria Using the Ribosomal Data Project Part 7 Individual Projects Individual Project 35 Hydrocarbon-Degrading Bacteria, Cleaning Up After Oil Spills Individual Project 36 Luminescent Bacteria: Bateria That Produce Light Appendix 1 Living Microorganisms (Bacteria, Fungi, Protozoa, and Helminths) Chosen for Study in this Manual Appendix 2 Dilution Practice Problems Appendix 3 Use of the Metric System with Conversions to the English System of Measurement Appendix 4 Alternative Procedures Appendix 5 Use of the Ocular Micrometer for Measurement of Relative and Absolute Cell Size Appendix 6 Use of the Hemocytometer for Determining Total Cell Number in a Liquid Suspension Appendix 7 History and Working Principles of Light Microscopy Appendix 8 Additional Reading