Nuclear Magnetic Resonance

This second volume in Modern Methods of Plant Analysis, New Series concentrates on nuclear magnetic resonance (NMR) spectroscopy, a powerful technique that is now being used for plant studies in a major way. This is the first time that a series of chapters by leading experts on the application of NMR to plant cells has been assembled in a single volume. The methods are described in detail so that even beginners can apply them directly in the laboratory. Topics include general metabolism in higher plants and fungi, intercellular pH, energy status, DNA structure analysis, multiple quantum two-dimensional NMR spectroscopy, chloroplast orientation, rubber analysis, and the use of NMR to determine pollen viability. This volume should provide not only an excellent practical guide to the possibilities of NMR application to the plant sciences, but also give impetus to its future use.

Contents:13C-NMR in Metabolic Studies / Determination of the Energy Status of Plant Cells by 31P-Nuclear Magnetic Resonance Spectroscopy / The Use of NMR Spectroscopy to Follow Deuterium in Studies of Fungal Metabolism / Proton NMR Studies on DNA Structure / NMR Methods for Determination of Intracellular pH / Orientation of Chloroplasts in Leaves by 1H NMR Spectroscopy / 13C-NMR Determination of Rubber Content in Guayule Bushes / Nuclear Magnetic Resonance and Pollen Quality / Subject Index.