Synopses & Reviews
Cryopreservation has proven to be an important tool for the storage and conservation of plant genetic resources as whole plants have been regenerated from isolated protoplasts, cells, tissues and organs cryogenically preserved in liquid nitrogen. The techniques of cryopreservation have developed to the extent where it is feasible to apply them to a diverse range of plant cells, tissues, and organs across a wide species range. These techniques are in increasing demand, but protocols are often not presented in an easily accessible format.
This book provides clearly written and illustrated protocols for direct use and elaborates on the critical points where problems may occur. It demonstrates how to employ cryopreservation in laboratories that are working in key plant science sectors: horticulture, biotechnology, agriculture, forestry, endangered species conservation, and germplasm preservation. This book is dedicated to assisting cryopreservation technology transfer both to laboratories unfamiliar with the techniques as well as those already involved in some form of cryopreservation.
Synopsis
to Cryopreservation.- Cryopreservation-Practical Considerations.- Cryopreservation Theory.- Development of PVS-Based Vitrification and Encapsulation-Vitrification Protocols.- Development of Encapsulation Dehydration.- Controlled Rate Cooling.- Cryopreservation Protocols for Working Laboratories.- Cryopreservation of Algae.- Cryopreservation of Bryophytes and Ferns.- Cryopreservation of Dedifferentiated Cell Cultures.- Cryopreservation of Embryogenic Cultures.- Cryopreservation of Excised Embryos and Embryonic Axes.- Cryopreservation of Monocots.- Cryopreservation of Herbaceous Dicots.- Cryopreservation of Temperate Berry Crops.- Cryopreservation of In Vitro Tissues of Deciduous Forest Trees.- Cryopreservation of Fruit and Ornamental Trees.- Cryopreservation of Dormant Buds.- Cryopreservation of Pollen.- Cryopreservation of Recalcitrant (i.e. Desiccation-Sensitive) Seeds.- Cryopreservation of Orthodox (Desiccation Tolerant) Seeds.
Synopsis
Plant Cryopreservation: A Practical Guide is a unique resource for plant scientists in many fields. This compilation provides over 100 ready-to-use protocols for plant types from algae and bryophytes to a range of flowering plants. It includes techniques for diverse plant parts such as dormant buds, pollen, and apical meristems, and for cell types such as suspension and callus cultures. Five introductory chapters describe theoretical principles, practical aspects of long-term cryopreserved storage, and details of the main cryopreservation techniques. The remaining 14 chapters are separated by plant type. Each chapter briefly reviews the literature and includes ready-to-use protocols designed for easy transfer into the lab and adaptation to new species.
Synopsis
Cryopreservation has proven to be an important tool for the storage and conservation of plant genetic resources. This book is a unique resource for plant scientists, providing more than 100 ready-to-use cryopreservation protocols for plant types from algae and bryophytes to a range of flowering plants. It includes techniques for diverse plant parts such as dormant buds, pollen, and apical meristems and for cell types such as suspension and callus cultures.
Table of Contents
Introduction.- History and Theoretical Principles.- Laboratory Facilities.- Cryopreservation of Plant Cells, Tissues and Organs.- Differentiated Tissues.- Vegetative Tissue.- Seeds (Orthodox, Intermediate, Recalcitrant).- Pollen.- Dormant Buds.- Associated Techniques.- Index.