Synopses & Reviews
This convenient volume presents, in a clear and readable fashion, a wide range of the latest techniques for protein structure studies. It provides the detailed coverage needed for immediate use in most laboratories, describing how to separate proteins into two dimensions, how to identify them immunochemically, and how to conduct procedures for characterizing interactions, such as ligand blotting, affinity labelling, and cross-linking. Completely up-to-date, the book includes special techniques for characterizing and purifying DNA-binding proteins, and practical guidelines for maintaining proteins in their active form are given.
Synopsis
Protein Structure: A Practical Approach gives general methods for determining protein structure that will be very widely applicable, from the simple electrophoretic, chromatographic, and spectrophotometric techniques to the relatively more complex and sophisticated methods of mass spectrometry and ultracentrifugation. This second edition has been fully updated to embrace the most recent developments in the field, and will be invaluable to all involved in protein research.
Synopsis
Protein Structure: A Practical Approach gives general methods for determining protein structure that will be very widely applicable, from the simple electrophoretic, chromatographic, and spectrophotometric techniques to the relatively more complex and sophisticated methods of mass spectrometry
and ultracentrifugation. This second edition has been fully updated to embrace the most recent developments in the field, and will be invaluable to all involved in protein research.
Table of Contents
1. Estimating Molecular Weights of Polypeptides by SDS Gel Electrophoresis, See
2. Isoelectric Focusing of Proteins in Conventional and Immobilized pH Gradients, Righetti
3. Two-Dimensional Polyacrylamide Gel Electrophoresis of Proteins, Strahler
4. Immunological Detection of Proteins of Known Sequence, Scheidtmann
5. Peptide Mapping, Carrey
6. Counting Integral Numbers of Residues by Chemical Modification, Hollecker
7. Disulphide Bonda Between Cysteine Residues, Creighton
8. Predictions of Protein Structure from Gene and Amino Acid Sequences, Argos
9. Folding Proteins, Jaenicke
10. Analysis of Protein Conformation by Gel Electrophoresis,Goldenberg
11. Spectral Methods of Characterizing Protein Conformation and Conformational Changes, Schmid
12. Immunochemical Analysis of Protein Conformation, Friguet
13. Measuring the Conformational Stability of a Protein, Pace
14. Stabilization of Protein Structure by Solvents, Timasheff
15. Minimizing Irreversible Modification of Proteins, Volkin