Synopses & Reviews
Display technologies have become a very powerful way of generating therapeutic lead molecules and specific reagents for increasing our understanding of biology; however, despite being first described shortly after phage display, the use of ribosome display and related methods have been much less widespread.
Synopsis
Display technologies are a powerful biological tool, yet the complexity of ribosome display methods has inhibited their use. This volume provides detailed and easily followed protocols with the aim of facilitating a greater uptake of these methods.
Synopsis
Part I: Review
1. Ribosome Display: A Perspective
Andreas Pl ckthun
Part II: Translation Extract Preparation
2. Preparation and Testing of E.coli S30 In Vitro Transcription Translation Extracts
James F. Zawada
Part III: Basic Ribosome Display and Related Selection Methods
3. Eukaryotic Ribosome Display Selection Using Rabbit Reticulocyte Lysate
Julie A. Douthwaite
4. Stabilized Ribosome Display for In Vitro Selection
Shuta Hara, Mingzhe Liu, Wei Wang, Muye Xu, Zha Li, and Yoshihiro Ito
5. Eukaryotic Ribosome Display with In Situ DNA Recovery
Mingyue He, Bryan M. Edwards, Damjana Kastelic, and Michael J. Taussig
6. mRNA Display Using Covalent Coupling of mRNA to Translated Proteins
Rong Wang, Steve W. Cotten, and Rihe Liu
7. SNAP Display: In Vitro Protein Evolution in Microdroplets
Miriam Kaltenbach and Florian Hollfelder
8. cDNA Display: Rapid Stabilization of mRNA Display
Shingo Ueno and Naoto Nemoto
Part IV: Applications of Ribosome Display Methods Using Natural Amino Acids
9. Optimization of Antibody Affinity by Ribosome Display Using Error-Prone or Site-Directed Mutagenesis
Leeanne Lewis and Chris Lloyd
10. Affinity Maturation of Phage Display Antibody Populations using Ribosome Display
Maria A. Groves and Adrian A. Nickson
11. Evolution of Protein Stability Using Ribosome Display
Andrew Buchanan
12. Selection of Lead Antibodies from Naive Ribosome Display Antibody Libraries
Peter Ravn
13. Evolution of Disulfide-Rich Peptide Aptamers Using cDNA Display
Yuki Mochizuki and Naoto Nemoto
14. Peptide Screening Using PURE Ribosome Display
Hiroyuki Ohashi, Takashi Kanamori, Eriko Osada, Bintang K. Akbar, and Takuya Ueda
15. Rapid Selection of High Affinity Binders Using Ribosome Display
Birgit Dreier and Andreas Pl ckthun
16. mRNA Display-Based Selections Using Synthetic Peptide and Natural Protein Libraries
Steve W. Cotten, Jianwei Zou, Rong Wang, Bao-cheng Huang, and Rihe Liu
17. Identification of Candidate Vaccine Genes Using Ribosome Display
Liancheng Lei
18. Ribosome Display for the Selection of Sac7d Scaffolds
Barbara Mouratou, Ghislaine B har, Lauranne Paillard-Laurance, St phane Colinet, and Fr d ric Pecorari
Part V: Incorporation of Non-Natural Amino Acids for Selection by Ribosome Display and Related Methods
19. Charging of tRNAs Using Ribozymes and Selection of Cyclic Peptides Containing Thioethers
Patrick C. Reid, Yuki Goto, Takayuki Katoh, and Hiroaki Suga
20. Update on Pure Translation Display with Unnatural Amino Acid Incorporation
R. Edward Watts and Anthony C. Forster
21. In Vitro Selection of Unnatural Cyclic Peptide Libraries Via mRNA Display
Zhong Ma and Matthew C.T. Hartman
Part VI: Case Studies
22. Optimization of CAT-354, a Therapeutic Antibody Directed Against Interleukin-13, Using Ribosome Display
George Thom and Ralph Minter
Table of Contents
Part I: Review 1. Ribosome Display: A Perspective Andreas Plückthun Part II: Translation Extract Preparation 2. Preparation and Testing of E.coli S30 In Vitro Transcription Translation Extracts James F. Zawada Part III: Basic Ribosome Display and Related Selection Methods 3. Eukaryotic Ribosome Display Selection Using Rabbit Reticulocyte Lysate Julie A. Douthwaite 4. Stabilized Ribosome Display for In Vitro Selection Shuta Hara, Mingzhe Liu, Wei Wang, Muye Xu, Zha Li, and Yoshihiro Ito 5. Eukaryotic Ribosome Display with In Situ DNA Recovery Mingyue He, Bryan M. Edwards, Damjana Kastelic, and Michael J. Taussig 6. mRNA Display Using Covalent Coupling of mRNA to Translated Proteins Rong Wang, Steve W. Cotten, and Rihe Liu 7. SNAP Display: In Vitro Protein Evolution in Microdroplets Miriam Kaltenbach and Florian Hollfelder 8. cDNA Display: Rapid Stabilization of mRNA Display Shingo Ueno and Naoto Nemoto Part IV: Applications of Ribosome Display Methods Using Natural Amino Acids 9. Optimization of Antibody Affinity by Ribosome Display Using Error-Prone or Site-Directed Mutagenesis Leeanne Lewis and Chris Lloyd 10. Affinity Maturation of Phage Display Antibody Populations using Ribosome Display Maria A. Groves and Adrian A. Nickson 11. Evolution of Protein Stability Using Ribosome Display Andrew Buchanan 12. Selection of Lead Antibodies from Naive Ribosome Display Antibody Libraries Peter Ravn 13. Evolution of Disulfide-Rich Peptide Aptamers Using cDNA Display Yuki Mochizuki and Naoto Nemoto 14. Peptide Screening Using PURE Ribosome Display Hiroyuki Ohashi, Takashi Kanamori, Eriko Osada, Bintang K. Akbar, and Takuya Ueda 15. Rapid Selection of High Affinity Binders Using Ribosome Display Birgit Dreier and Andreas Plückthun 16. mRNA Display-Based Selections Using Synthetic Peptide and Natural Protein Libraries Steve W. Cotten, Jianwei Zou, Rong Wang, Bao-cheng Huang, and Rihe Liu 17. Identification of Candidate Vaccine Genes Using Ribosome Display Liancheng Lei 18. Ribosome Display for the Selection of Sac7d Scaffolds Barbara Mouratou, Ghislaine Béhar, Lauranne Paillard-Laurance, Stéphane Colinet, and Frédéric Pecorari Part V: Incorporation of Non-Natural Amino Acids for Selection by Ribosome Display and Related Methods 19. Charging of tRNAs Using Ribozymes and Selection of Cyclic Peptides Containing Thioethers Patrick C. Reid, Yuki Goto, Takayuki Katoh, and Hiroaki Suga 20. Update on Pure Translation Display with Unnatural Amino Acid Incorporation R. Edward Watts and Anthony C. Forster 21. In Vitro Selection of Unnatural Cyclic Peptide Libraries Via mRNA Display Zhong Ma and Matthew C.T. Hartman Part VI: Case Studies 22. Optimization of CAT-354, a Therapeutic Antibody Directed Against Interleukin-13, Using Ribosome Display George Thom and Ralph Minter 23. Affinity Maturation and Functional Dissection of a Humanized Anti-RAGE Monoclonal Antibody by Ribosome Display Simon E. Hufton