Synopses & Reviews
The idea that the immune system may play a role in protecting against malignant disease is not new. Since the earliest days of immunology research, scientists have sought to understand the mechanisms by which the host immune system might recognize cancer cells, and to manipulate these responses for therapeutic gain. It has become clear that the key to immune recognition of tumors lies in the relationship between cells in the microenvironment of the tumor itself. This volume provides a comprehensive collection of techniques for establishing tumor cell lines, testing their immunogenicity, and characterizing the major effector cells, leading to protocols for manipulating antibody and T-cell receptor structure, preparing cytokine-secreting tumor cells and testing the efficiency of these new agents in preventing tumor growth. Researchers in immunology as a whole in addition to those interested in cancer immunology will find this volume to be an invaluable practical resource.
Table of Contents
1. Preparation of tumour cell-lines,
A.P. Wilson2. Assessment of the immunogenicity of tumour models, R.R. Miller, B.E. Miller, and G.H. Heppner
3. Flow cytometry of lymphocytes and tumour cells, J. Lawry
4. (Glyco-) protein antigens and peptide epitopes of tumours, M.R. Price
5. Isolation and characterization of natural killer cells, T. Timonen, A. Maenpaa, and P. Kovanen (with additional material by V.P. Thorgersson and A.R. Mackay)
6. Isolation and characterization of mononuclear phagocytes, L.J. Partridge and I. Dransfield
7. T-cells: proliferative and cytotoxic responses, R.A. Robins
8. Cloning and propagation of human T lymphocytes, G. Pawelec
9. Association of TCR expression with MHC recognition, S.L. Hand, B.L. Hall, and O.J. Finn
10. Cytokines: identification and measurement of gene activation, F.S. di Giovine, S. Stones, D. Wojtacha, and G.W. Duff
n 11 Cytokine bioassay, F.P. Winstanley
12. Strategies for the production of human monoclonal antibodies, M. Kurpisz and G. Wilson
13. Radiolabelling antibodies for imaging and targeting, M.V. Pimm and S.J. Gibbon
14. Preparation of multispecific F(ab) and F(ab) antibody derivatives, M.J. Glennie, A.L. Tutt, and J. Greenman
15. Construction and expression of Ig fusion proteins, M.S. Hayden, H.P. Fell, and N.F. Landolfi
16. Reshaping human monoclonal antibodies for in vivo diagnosis and therapy, M.E. Verhoeyen
17. Single-chain Fvs, M. Whitlow and D. Filpula
18. Engineering cytokine-secreting tumour cells, P.M. Patel, C.L. Flemming, S.A. Eccles, and M.K.L. Collins
19. Ex vivo activation of effector cells, K.E. Platts
20. Genetic imortalization of human lymphocytes using retroviral vectors, C. Darnbrough
21. Modifying the specificity of T cells using chimeric Ig/TCR genes, Z. Eshhar, G. Gross, and J. Treisman
22. The use of pre-clinical rodent models for cancer immunotherapy, R.B. Wiltrout
23. Use of the tumour spheroid model in immunotherapy, I.E. Grcia de Palazzo, M. Holmes, K. Alpaugh, and L.M. Weiner