Synopses & Reviews
The zebrafish has become one of the most important model organisms to study biological processes within a living body. As a vertebrate that has many of the strengths of invertebrate model systems, it offers numerous advantages to researchers interested in many aspects of embryonic development, physiology and disease. This book not only provides a complete set of instructions that will allow researchers to establish the zebrafish in their laboratory. It also gives a broad overview of commonly used methods and a comprehensive collection of protocols describing the most powerful techniques.
Table of Contents
List of protocols
Abbreviations
Introduction: zebrafish as a system to study development and organogenesis, Christiane Nüsslein-Volhard, Darren T. Gilmour, and Ralf Dahm
1. Keeping and raising zebrafish, Michael Brand, Michael Granato, and Christiane Nüsslein-Volhard
2. Looking at embryos, Stefan Schulte-Merker
3. The morphology of larval and adult zebrafish, Thomas F. Schilling
4. Cell labelling and transplantation techniques, Donald A. Kane and Yasuyuki Kishimoto
5. Manipulating gene expression in the zebrafish, Darren T. Gilmour, Jason R. Jessen, and Shuo Lin
6. Mutagenesis, Francisco Pelegri
7. Mapping and cloning, Robert Geisler
Appendix 1. List of suppliers
Appendix 2. Atlas of embryonic stages of development in
Appendix 3. Table of zebrafish mutations
Index